Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Effect of different concentrations of Ang II on migration of human bone marrow MSCs. a Nondirectional migration ability of human bone marrow MSCs after stimulations with different concentrations of Ang II (10 –8 , 10 –7 , 10 –6 , 10 –5 , and 3 × 10 –5 M) examined using the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of human bone marrow MSCs after stimulations with the different concentrations of Ang II indicated examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. n = 3; * p < 0.05 vs normal. Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Effect of AT1R and AT2R antagonists on Ang II-mediated migration of MSCs. a Nondirectional migration ability of MSCs after stimulation with 100 nM Ang II following pretreatment with Losartan (5 μM) and/or PD-123319 (5 μM) examined with the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of MSCs after stimulation with 100 nM Ang II following pretreatment with Losartan (5 μM) and/or PD-123319 (5 μM) examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. Significant differences were found compared with the normal group ( n = 3; * p < 0.05) and compared with the control group ( n = 3; # p < 0.05). Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy, Control

Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Roles of FAK and Rho GTPases in the migration of MSCs. a Nondirectional migration ability of MSCs after stimulation with Ang II and/or inhibitors of FAK and the Rho GTPases examined using the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of MSCs after stimulation with Ang II and/or inhibitors of FAK and the Rho GTPases examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. n = 3; * p < 0.05 vs normal, # p < 0.05 vs the control group. Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy, Control

Journal: PLoS ONE

Article Title: Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays

doi: 10.1371/journal.pone.0201438

Figure Lengend Snippet: Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to Transwell exposure to D . tertiolecta .

Article Snippet: The ichthyotoxic microalga ( H . akashiwo ) was co-cultured with the CHSE-214 cell line by using a Transwell culture system with 0.4 μm pore polyester membrane inserts (Corning HTS Transwell).

Techniques: Expressing